منابع مشابه
Organ and cellular localization of asparagine synthetase in rice plants.
DNA gel blot analysis suggested that asparagine synthetase (AS; EC 6.3.5.4) occurred as a single gene in rice. A fusion protein consisting of 17 kDa tagged-region from pET32a(+) expression plasmid and 42 kDa N-terminal region of rice AS was first expressed in Escherichia coli. The resulting polypeptide was purified and a mono-specific antibody for rice AS was prepared after affinity-purificatio...
متن کاملAsparagine Metabolic Pathways in Arabidopsis.
Inorganic nitrogen in the form of ammonium is assimilated into asparagine via multiple steps involving glutamine synthetase (GS), glutamate synthase (GOGAT), aspartate aminotransferase (AspAT) and asparagine synthetase (AS) in Arabidopsis. The asparagine amide group is liberated by the reaction catalyzed by asparaginase (ASPG) and also the amino group of asparagine is released by asparagine ami...
متن کاملAsparagine formation in soybean nodules.
(15)NH(4) (+) and [(15)N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of (15)NH(4) (+) - feeding, the (15)N abundance ratio was highest in the amide nitrogen of glutamine, followed by glu...
متن کاملAsparagine synthetase in corn roots.
The level of asparagine synthetase is low in 10-mm root tips from corn seedings (Zea mays W64 x W182F) but relatively high in mature root sections taken 20 to 35 mm from the tip. When root tips are excised there is a marked increase in asparagine synthetase over a 5-hour period. In mature root sections, on the other hand, the asparagine synthetase activity declines over the same 5-hour period. ...
متن کاملAsparagine biosynthesis in soybean nodules.
Asparagine biosynthesis in soybean (Glycine max [L.] Merr.) nodules has been difficult to demonstrate due to the poor conversion of suspected immediate precursors to asparagine and the instability of the key enzyme asparagine synthetase. The present study was designed to explore the effects of two ammonium assimilation inhibitors on the metabolism of (14)CO(2) to [(14)C]asparagine and to demons...
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ژورنال
عنوان ژورنال: Annals of Applied Biology
سال: 2007
ISSN: 0003-4746,1744-7348
DOI: 10.1111/j.1744-7348.2006.00104.x